Human Phospho-STAT2 (Y690) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2890
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Flow Cytometry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1021D
Product Specifications
Immunogen
Phosphopeptide containing the human STAT2 Y690 site
Specificity
Detects human phospho-STAT2 (Y690) in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-STAT2 (Y690) Antibody
Detection of Human Phospho-STAT2 (Y690) by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-STAT2 (Y690) at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of STAT2 in Daudi Human Cell Line by Flow Cytometry.
Daudi human Burkitt's lymphoma cell line untreated (open histogram) or treated with 500 U/mL Recombinant Human IFN-aA (11100-1, filled histogram) for 20 minutes was stained with Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890), followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with 90% methanol.
Detection of Human Phospho-STAT2 (Y690) by Simple WesternTM.
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT2 (Y690) at approximately 111 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Phospho-STAT2 (Y690) Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1) were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol
Simple Western
0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1)
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1)
Western Blot
0.05 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1)
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT2
STAT2 (signal transducer and activator of transcription 2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha- and beta-) interferon signaling. All STATs contain an N-terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.
Long Name
Signal Transducer and Activator of Transcription 2
Alternate Names
interferon alpha induced transcriptional activator, ISGF-3, MGC59816, p113, signal transducer and activator of transcription 2, signal transducer and activator of transcription 2, 113kD, signal transducer and activator of transcription 2, 113kDa, STAT113
Gene Symbol
STAT2
Additional STAT2 Products
Product Documents for Human Phospho-STAT2 (Y690) Antibody
Product Specific Notices for Human Phospho-STAT2 (Y690) Antibody
For research use only
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