Human Phospho-STAT3 (S727) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4934
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 788335
Product Specifications
Immunogen
Phosphopeptide containing the human STAT3 S727 site
Specificity
Detects human STAT3 when phosphorylated at S727.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Phospho-STAT3 (S727) Antibody
Detection of Human Phospho-STAT3 (S727) by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-STAT3 (S727) Monoclonal Antibody (Catalog # MAB4934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-STAT3 (S727) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Phospho-STAT3 (S727) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry.
Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Mouse anti-Human Phospho-STAT3 (S727) Molyclonal Antibody (Catalog # MAB4934) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
Detection of Human STAT3 by Western Blot
Regulation of hepcidin by IL-10 in primary macrophages, showing (a) the effect of IL-10 on Hepcidin mRNA synthesis with and without anti-IL-10 blocking antibody; (b) Western blot of STAT3 phosphorylation 20 minutes after exposure to increasing concentrations of IL-10; (c) STAT3-P inhibition of IL-10 (30 ng/mL) by Stattic.Bar plots show data from at least 3 independent experiments and the sho Western blot shows data from a representative experiment. M = media, iso ctrl = isotype control. * P<0.05, ***P<0.01 (Mann-Whitney test, compared with Media control). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24520384), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Phospho-STAT3 (S727) Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: IFN alpha-treated Daudi Human Cell Line fixed with paraformaldehyde and permeabilized with methanol
Sample: IFN alpha-treated Daudi Human Cell Line fixed with paraformaldehyde and permeabilized with methanol
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human IL‑6 (Catalog # 206-IL)
Sample: HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human IL‑6 (Catalog # 206-IL)
Reviewed Applications
Read 1 review rated 5 using MAB4934 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT3
Long Name
Signal Transducer and Activator of Transcription 3
Alternate Names
Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)
Gene Symbol
STAT3
Additional STAT3 Products
Product Documents for Human Phospho-STAT3 (S727) Antibody
Product Specific Notices for Human Phospho-STAT3 (S727) Antibody
For research use only
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