Human Phospho-STAT3 (Y705) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4607
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Rabbit
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 805435
Product Specifications
Immunogen
Phosphopeptide containing the human STAT3 Y705 site
Specificity
Detects human STAT3 when phosphorylated at Y705.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human Phospho-STAT3 (Y705) Antibody
Detection of Human Phospho‑STAT3 (Y705) by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes or 50 µg/mL Recombinant Human IL-6 (Catalog # 206-IL) for 15 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB4607) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-STAT3 (Y705) Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1) and HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human IL‑6 (Catalog # 206-IL)
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alphaA (Catalog # 11100-1) and HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human IL‑6 (Catalog # 206-IL)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT3
Human STAT-3/STAT3a (signal transducer and activator of transcription 3; also called APRF) is a 90-95 kDa member of the STAT family of transcription factors. It is 770 amino acids (aa) in length, contains one SH2 domain (aa 580-670), and is found in almost all cell types. STAT3 mediates gp130, LIF-R, OB-R, IL10-R and EGFR signaling. Upon activation, receptors such as gp130 phosphorylate STAT3 at Ser727, which results in transcriptional activation. Phosphorylation at Tyr705 mediates homo- and hetero-dimerization with STAT1 and nuclear translocation, and is an important event in oncogenic transformation. The STAT3b isoform shows a six aa substitution for aa 716-770, thus eliminating the Ser727 site. Human and mouse STAT3a share 99% aa sequence identity.
Long Name
Signal Transducer and Activator of Transcription 3
Alternate Names
Acute-phase response factor, APRFMGC16063, DNA-binding protein APRF, FLJ20882, HIES, signal transducer and activator of transcription 3, signal transducer and activator of transcription 3 (acute-phase responsefactor)
Gene Symbol
STAT3
Additional STAT3 Products
Product Documents for Human Phospho-STAT3 (Y705) Antibody
Product Specific Notices for Human Phospho-STAT3 (Y705) Antibody
For research use only
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