Human Phospho-STAT4 (Y693) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4319
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human Phospho-STAT4 Y693 site
Specificity
Detects human STAT4 when phosphorylated at Y693.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-STAT4 (Y693) Antibody
Detection of Human Phospho-STAT4 (Y693) by Western Blot.
Western blot shows lysates of NC-37 human Burkitt's lymphoma B lymphoblast cell line untreated (-) or treated (+) with pervanadate for 5 minutes. PVDF membrane was probed with 1 µg/mL Human Phospho-STAT4 (Y693) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4319) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT4 (Y693) was detected at approximately 95 kDa (as indicated). For additional reference, duplicate lysates were probed with 1.0 µg/mL Human/Mouse STAT4 Polyclonal Antibody (lower panel, Catalog # PA-ST4). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-STAT4 (Y693) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: NC‑37 human Burkitt's lymphoma B lymphoblast cell line treated with pervanadate
Sample: NC‑37 human Burkitt's lymphoma B lymphoblast cell line treated with pervanadate
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT4
Long Name
Signal Transducer and Activator of Transcription 4
Alternate Names
signal transducer and activator of transcription 4, SLEB11
Gene Symbol
STAT4
Additional STAT4 Products
Product Documents for Human Phospho-STAT4 (Y693) Antibody
Product Specific Notices for Human Phospho-STAT4 (Y693) Antibody
For research use only
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