Phosphopeptide containing human STAT5b Y699 site (amino acid sequence of this peptide is identical to a corresponding region of human STAT5a containing Y694)
Accession # P51692

Specificity

Detects human STAT5a/b when phosphorylated at Y964/Y699 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Detection of Human Phospho-STAT5a/b (Y694/Y699) by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (Catalog # 11100-1) for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-STAT5a/b (Y694/Y699) Monoclonal Antibody (Catalog # MAB41901) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT5a/b at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Phospho-STAT5a/b (Y694/Y699) in IFN alpha-treated Daudi Human Cell Line by Flow Cytometry.

Daudi human Burkitt's lymphoma cell line was unstimulated (open histogram) or treated with 500 U/mL rhIFN-alpha for 20 minutes (filled histogram) and stained with Rabbit anti-Human Phospho-STAT5a/b (Y694/Y699) Molyclonal Antibody (Catalog # MAB41901) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

Applications for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: IFN alpha-treated Daudi Human Cell Line fixed with paraformaldehyde and permeabilized with methanol

Western Blot

0.1 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB41901 in the following applications:

Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT5a/b

Signal transduction and activator of transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL-22, IL-6, IFN-alpha). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN₃GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation, and survival.

Long Name

Signal Transducer and Activator of Transcription 5b

Alternate Names

MGF, STAT5, STAT5A signal transducer and activator of transcription 5A

UniProt

P51692

Additional STAT5a/b Products

Product Documents for Human Phospho-STAT5a/b (Y694/Y699) Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Phospho-STAT5a/b (Y694/Y699) Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Phospho-STAT5a/b (Y694/Y699) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB41901

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all STAT5a/b Antibodies »

Product Specifications

Immunogen