Human Phospho-TOR (S2448) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1665

R&D Systems, part of Bio-Techne
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MAB1665
MAB1665-SP
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Infared Microscopy, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 834115

View all TOR Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human TOR S2448 site
Accession # P42345

Specificity

Detects human Phospho-TOR (S2448) in ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Phospho-TOR (S2448) Antibody

Detection of Human Phospho-TOR (S2448) antibody by Western Blot.

Detection of Human Phospho-TOR (S2448) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line treated (+) with 1 µM Camptothecin (CPT) for 5 hours. PVDF membrane was treated (+) or untreated (-) with 300 U/mL CIP for 1 hour and probed with 1 µg/mL of Rat Anti-Human Phospho-TOR (S2448) Monoclonal Antibody (Catalog # MAB1665) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-TOR (S2448) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-TOR (S2448) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Camptothecin (CPT) and CIP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TOR

The mammalian Target of Rapamycin (mTOR) is a member of the PI 3-kinase-related kinase (PIKK) family. mTOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. Recent evidence suggests that mTOR is phosphorylated at S2448 by the S6 protein kinase 1 (S6K1) (1, 2), rather than a direct phosphorylation by the Akt protein kinase. This S2448 phosphorylation activates mTOR to affect the downstream control of cell growth and survival.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Entrez Gene IDs

2475 (Human); 287871 (Rat)

Gene Symbol

MTOR

UniProt

P42345

Additional TOR Products

Product Documents for Human Phospho-TOR (S2448) Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Phospho-TOR (S2448) Antibody

For research use only

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