Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4170
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Cell Culture, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human VEGFR1/Flt-1 Y1213 site
Specificity
Detects human VEGFR1/Flt-1 when phosphorylated at Y1213.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody
Detection of Human Phospho-VEGFR1/Flt‑1 (Y1213) by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cell untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Phospho-VEGFR1/Flt-1 (Y1213) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4170), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-VEGFR1/Flt-1 (Y1213) at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Pervanadate-treated HUVEC human umbilical vein endothelial cells
Sample: Pervanadate-treated HUVEC human umbilical vein endothelial cells
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGFR1/Flt-1
VEGFR1, also known as Flt-1, is a receptor tyrosine kinase that contains seven Ig-like repeats in its extracellular domain, a single transmembrane region, and a kinase domain in the intracellular region. Ligand binding induces autophosphorylation on multiple intracellular tyrosine residues. Y1213 is a major binding site for PI3K, Nck, and SHP-2.
Long Name
Vascular Endothelial Growth Factor Receptor 1
Alternate Names
Flt-1, FLT1, FRT, VEGF R1, VEGFR-1
Gene Symbol
FLT1
Additional VEGFR1/Flt-1 Products
Product Documents for Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody
Product Specific Notices for Human Phospho-VEGFR1/Flt-1 (Y1213) Antibody
For research use only
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