Human PI 3-Kinase p110 beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2686
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 269020
Product Specifications
Immunogen
E. coli-derived recombinant human PI 3-Kinase p110 beta
Met152-Val349
Accession # P42338
Met152-Val349
Accession # P42338
Specificity
Detects endogenous human PI 3-kinase p110 beta in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PI 3-Kinase p110 beta Antibody
Detection of Human PI 3-Kinase p110 beta by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110 beta Monoclonal Antibody (Catalog # MAB2686) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p110 beta at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human PI 3-Kinase p110 beta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Sample: A431 human epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4 using MAB2686 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p110 beta
Class IA phosphoinositide 3-kinases (PI 3-kinases) are a family of p85/p110 heterodimeric lipid kinases that function as signal transducers downstream of growth factor receptors, mainly receptor tyrosine kinases. Mammals express three class IA catalytic subunits: p110 alpha, p110 beta, and p110 delta. Reports suggest that the p110 beta subunit may have special importance in promoting cell proliferation.
Long Name
Protein Tyrosine Phosphatase 1B
Alternate Names
PI 3Kinase p110 beta, PIK3CB
Entrez Gene IDs
5291 (Human)
Gene Symbol
PIK3CB
UniProt
Additional PI 3-Kinase p110 beta Products
Product Documents for Human PI 3-Kinase p110 beta Antibody
Product Specific Notices for Human PI 3-Kinase p110 beta Antibody
For research use only
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