Human PI 3-Kinase p110 gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2999
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse, Lobster
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 304103
Product Specifications
Immunogen
E. coli-derived recombinant human PI 3-Kinase p110 gamma
Lys298-Leu467
Accession # P48736
Lys298-Leu467
Accession # P48736
Specificity
Detects endogenous human PI 3-Kinase p110 gamma in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human PI 3-Kinase p110 gamma Antibody
Detection of Human PI 3-Kinase p110 gamma by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human PI 3-Kinase p110 gamma Monoclonal Antibody (Catalog # MAB2999) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PI 3-Kinase p110 gamma at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PI 3-Kinase p110 gamma Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line
Sample: K562 human chronic myelogenous leukemia cell line and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p110 gamma
Class I phosphoinositide 3-kinases (PI 3-Kinases) are dual-specific lipid and protein kinases involved in numerous intracellular signaling pathways. Class IA PI 3‑Kinases (p110 alpha, beta and delta) are typically activated through receptor tyrosine kinase signaling, whereas p110 gamma, the single member of class IB, is activated by seven‑transmembrane G‑protein‑coupled receptors (GPCRs). While p110 alpha and beta are ubiquitiously expressed, expression of p110 gamma is mainly restricted to cells of the hematopoietic system.
Long Name
Protein Tyrosine Phosphatase 1G
Alternate Names
p120-PI3K, PI 3Kinase p110 gamma, PI3CG, PI3K-gamma, PIK3CG
Gene Symbol
PIK3CG
UniProt
Additional PI 3-Kinase p110 gamma Products
Product Documents for Human PI 3-Kinase p110 gamma Antibody
Product Specific Notices for Human PI 3-Kinase p110 gamma Antibody
For research use only
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