Human PIEZO1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11557
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1081418
Product Specifications
Immunogen
PIEZO1 containing synthetic peptide
Accession # Q92508
Accession # Q92508
Specificity
Detects a synthetic peptide around aa 150 in Direct ELISA
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PIEZO1 Antibody
Detection of PIEZO1 in Human Kidney.
PIEZO1 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human PIEZO1 Monoclonal Antibody (Catalog # MAB11557) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PIEZO1 Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIEZO1
References
- Zhao X, Kong Y, Liang B, Xu J, Lin Y, Zhou N, Li J, Jiang B, Cheng J, Li C, Wang W. Mechanosensitive Piezo1 channels mediate renal fibrosis. JCI Insight. 2022 Apr 8;7(7):e152330. doi: 10.1172/jci.insight.152330. PMID: 35230979; PMCID: PMC9057604.
- Carrisoza-Gaytan R, Kroll KT, Hiratsuka K, Gupta NR, Morizane R, Lewis JA, Satlin LM. Functional maturation of kidney organoid tubules: PIEZO1-mediated Ca2+ signaling. Am J Physiol Cell Physiol. 2023 Mar 1;324(3):C757-C768. doi: 10.1152/ajpcell.00288.2022. Epub 2023 Feb 6. PMID: 36745528; PMCID: PMC10027089.
Long Name
Piezo-type mechanosensitive ion channel component 1
Alternate Names
FAM38A, KIAA0233, Mib, Protein FAM38A
Gene Symbol
PIEZO1
UniProt
Additional PIEZO1 Products
Product Documents for Human PIEZO1 Antibody
Product Specific Notices for Human PIEZO1 Antibody
For research use only
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