Human PIK3R4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6104
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 576514
Product Specifications
Immunogen
E. coli-derived recombinant human PIK3R4
Gln58-Arg230
Accession # Q99570
Gln58-Arg230
Accession # Q99570
Specificity
Detects human PIK3R4 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PIK3R4 Antibody
Detection of Human PIK3R4 by Western Blot.
Western blot shows lysates of TT human medullary thyroid cancer cell line and MO7e human megakaryocytic leukemic cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human PIK3R4 Monoclonal Antibody (Catalog # MAB6104) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PIK3R4 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PIK3R4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: TT human medullary thyroid cancer cell line and MO7e human megakaryocytic leukemic cell line
Sample: TT human medullary thyroid cancer cell line and MO7e human megakaryocytic leukemic cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB6104 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIK3R4
PIK3R4 (phosphoinositide 3-kinase regulatory subunit 4) is a 150 kDa, 1358 amino acid (aa) adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway. It is a regulatory subunit of the PI3K complex and is ubiquitously expressed. Within aa 58‑230, human PIK3R4 shares 99% aa identity with mouse and rat PIK3R4.
Long Name
Phosphoinositide-3-kinase, Regulatory Subunit 4
Alternate Names
p150, VPS15
Gene Symbol
PIK3R4
UniProt
Additional PIK3R4 Products
Product Documents for Human PIK3R4 Antibody
Product Specific Notices for Human PIK3R4 Antibody
For research use only
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