Human PIK3R5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6388
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 601214
Product Specifications
Immunogen
E. coli-derived recombinant human PIK3R5
Lys530-Pro726
Accession # Q8WYR1
Lys530-Pro726
Accession # Q8WYR1
Specificity
Detects human PIK3R5 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PIK3R5 Antibody
Detection of Human PIK3R5 by Western Blot.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human PIK3R5 Monoclonal Antibody (Catalog # MAB6388) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PIK3R5 at approximately 97 kDa and the spliced isoform at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human PIK3R5 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line
Sample: MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIK3R5
Phosphoinositide 3-kinase regulatory subunit 5 (PIK3R5; also PI3-kinase p101 subunit, Ptdlns-3-kinase p101, and p101-PI3K) is a 97 kDa regulatory subunit of the PI3K gamma complex. It is expressed as a heterodimer with the catalytic subunit PIK3CG/p120. Human PIK3R5 is 880 amino acids (aa) in length. The heterodimerization region is made up of aa 25-101, and aa 653-753 comprise the region for interaction with G beta gamma proteins. A second 55 kDa isoform is formed by the deletion of aa 1-386. Human PIK3R5 is 86% aa identical to mouse PIK3R5. PIK3R5 is highly expressed in leukocytes, followed by spleen, lymph node, thymus, and bone marrow.
Long Name
Phosphoinositide-3-kinase, Regulatory Subunit 5
Alternate Names
FOAP-2, p101-PI3K
Gene Symbol
PIK3R5
UniProt
Additional PIK3R5 Products
Product Documents for Human PIK3R5 Antibody
Product Specific Notices for Human PIK3R5 Antibody
For research use only
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