Human PILR-alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6484
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PILR-alpha
Gln20-Thr196
Accession # Q9UKJ1
Gln20-Thr196
Accession # Q9UKJ1
Specificity
Detects human PILR-alpha in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PILR-alpha Antibody
Detection of PILR‑ alpha in Human peripheral blood granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Sheep Anti-Human PILR-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6484, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).Applications for Human PILR-alpha Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood granulocytes
Sample: Human peripheral blood granulocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PILR-alpha
PILR-alpha (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14+CD1a- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-alpha acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.
Long Name
Paired-Ig-likeType 2 Receptor alpha
Alternate Names
FDF03, PILRA, PILRalpha
Gene Symbol
PILRA
UniProt
Additional PILR-alpha Products
Product Documents for Human PILR-alpha Antibody
Product Specific Notices for Human PILR-alpha Antibody
For research use only
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