Human PINK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4357
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 420920
Product Specifications
Immunogen
E. coli-derived recombinant human PINK1
Met1-Leu581
Accession # ABM87645
Met1-Leu581
Accession # ABM87645
Specificity
Detects human PINK1 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human PINK1 Antibody
PINK1 in Human Brain.
PINK1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Rat Anti-Human PINK1 Monoclonal Antibody (Catalog # MAB4357) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PINK1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PINK1
PTEN-induced putative kinase 1 (PINK1) is a ubiquitous protein with highest levels in heart, skeletal muscle, and testis, and with lower expression in other tissues, including brain. PINK1 contains a mitochondrial targeting motif and a highly conserved protein kinase domain with strong homology to serine/threonine kinases of the calcium/calmodulin family. Mutations in the Pink1 gene have been identified as a cause of autosomal recessive early-onset parkinsonism.
Long Name
PTEN-induced Putative Kinase 1
Alternate Names
BRPK, PARK6
Gene Symbol
PINK1
UniProt
Additional PINK1 Products
Product Documents for Human PINK1 Antibody
Product Specific Notices for Human PINK1 Antibody
For research use only
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