Human PKC epsilon Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5134

R&D Systems, part of Bio-Techne
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MAB5134
MAB5134-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 666823

View all PKC epsilon Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC epsilon
Gln580-Pro737
Accession # Q02156

Specificity

Detects human PKC epsilon in direct ELISAs. In direct ELISAs, less than 20% cross-reactivity with recombinant human (rh) PKC delta and rhPKC iota is observed. No cross-reactivity with rhPKC alpha, beta1, beta2, gamma, eta, μ, nu, theta, or zeta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PKC epsilon Antibody

PKCe antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P).

PKC epsilon in Human Lung Cancer Tissue.

PKCe was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human PKCe Monoclonal Antibody (Catalog # MAB5134) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human PKC epsilon Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC epsilon

PKC epsilon (protein kinase C-epsilon) is an 87 kDa member of the novel PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a widely-expressed Ca++‑insensitive, phospholipid-dependent enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC epsilon is 737 amino acids (aa) in length. It contains two general regions: a non-Ca++-binding plus lipid binding regulatory region (aa 1-99 and 169-292, respectively), and an ATP-binding catalytic domain (aa 408-668). Phosphorylation of PKC epsilon on Thr566, Ser368 and Ser729 activates the enzyme and increases its molecular weight to 92 kDa. One potential splice form shows a 114 aa substitution for aa 118-737. Over aa 580-737, human PKC epsilon is 98% and 99% aa identical to mouse and canine PKC epsilon, respectively.

Long Name

Protein Kinase C epsilon

Alternate Names

PKCE, PRKCE

Entrez Gene IDs

5581 (Human); 18754 (Mouse); 29340 (Rat)

Gene Symbol

PRKCE

UniProt

Q02156

Additional PKC epsilon Products

Product Documents for Human PKC epsilon Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PKC epsilon Antibody

For research use only

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