Human PKC epsilon Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB51341
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 666843
Product Specifications
Immunogen
E. coli-derived recombinant human PKC epsilon
Gln580-Pro737
Accession # Q02156
Gln580-Pro737
Accession # Q02156
Specificity
Detects human PKC epsilon in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PKC epsilon Antibody
Detection of Human PKC epsilon by Western Blot.
Western blot shows lysates of Human brain (hippocampus) tissue and human brain (hypothalamus) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PKCe Monoclonal Antibody (Catalog # MAB51341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKCe at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PKC epsilon by Simple WesternTM.
Simple Western shows lysates of human hippocampus, loaded at 0.5 mg/ml. A specific band was detected for PKC epsilon at approximately 98 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human PKC epsilon Monoclonal Antibody (Catalog # MAB51341). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.Applications for Human PKC epsilon Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Human hippocampus
Sample: Human hippocampus
Western Blot
2 µg/mL
Sample: Human brain (hippocampus) tissue and human brain (hypothalamus) tissue
Sample: Human brain (hippocampus) tissue and human brain (hypothalamus) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC epsilon
Long Name
Protein Kinase C epsilon
Alternate Names
PKCE, PRKCE
Gene Symbol
PRKCE
UniProt
Additional PKC epsilon Products
Product Documents for Human PKC epsilon Antibody
Product Specific Notices for Human PKC epsilon Antibody
For research use only
Loading...
Loading...
Loading...
Loading...