Jurkat human acute T cell leukemia cell line was stained with Human PKC theta Monoclonal Antibody (Catalog # MAB4368, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Detection of Human PKC theta by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human PKC theta Monoclonal Antibody (Catalog # MAB4368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKC theta at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Detection of Human PKC theta by Simple Western^TM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKC theta at approximately 82 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human PKC theta Monoclonal Antibody (Catalog # MAB4368). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human PKC theta Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with methanol

Simple Western

5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB4368 in the following applications:

Antibody microarray (1 Review)
Microarray (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC theta

PKC theta (PRKCQ) is a calcium-independent phospholipid-dependent serine and threonine kinase that belongs to the novel PKC subfamily. PKC theta plays a critical role in several aspects of T cell biology, and knockout mice develop autoimmune disease. PKC theta functions through the activation of NF kappaB and AP1 transcription factors.

Long Name

Protein Kinase C theta

Alternate Names

PRKCQ, PRKCT

Entrez Gene IDs

5588 (Human); 18761 (Mouse); 85420 (Rat)

Gene Symbol

PRKCQ

UniProt

Q04759

Additional PKC theta Products

Product Documents for Human PKC theta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PKC theta Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human PKC theta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4368

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PKC theta Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PKC theta Antibody

Detection of PKC theta in Jurkat Human Cell Line by Flow Cytometry.