Human Plasminogen Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1939
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Neutralization
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 270409
Product Specifications
Immunogen
Human plasma-derived Plasminogen
Glu20-Asn810
Glu20-Asn810
Specificity
Detects human PLG, human Plasmin, and the catalytic domain in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Plasminogen Antibody
Neutralization of Plasminogen Activity by Human Plasminogen Antibody.
The cleavage of Suc-AFK-AMC (100 µM) by Human Plasminogen (1 µg/mL, Catalog # 1939-SE) is measured after preincubation with increasing concentrations of Mouse Anti-Human Plasminogen Monoclonal Antibody (Catalog # MAB1939). The ND50 is typically 1 µg/mL.Applications for Human Plasminogen Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Human Plasminogen (Catalog # 1939-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Human Plasminogen (Catalog # 1939-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Human Plasminogen (1 µg/mL, Catalog # 1939-SE) cleavage of the fluorogenic peptide substrate Suc-AFK-Amc (100 µM). The Neutralization Dose (ND50) is typically 1 µg/mL.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plasminogen
Plasminogen (PLG) is the precursor of plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation, and tumor invasion (1, 2). Synthesized in the kidney, PLG is found in plasma and many extracellular fluids. Activated by u- or t-plasminogen activator, the single-chain PLG (amino acid residues 20-810) is converted to plasmin, which consists of disulfide bond-linked heavy chain A (residues 20-580) and light chain B (residues 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named as angiostatin, a novel angiogenesis inhibitor (3, 4).
References
- Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
- Forsgren, M. et al. (1987) FEBS Lett. 213:254.
- O’Reilly, M.S. et al. (1994) Cell 79:315.
- Sim, B.K. et al. (1997) Cancer Res. 57:1329.
Alternate Names
Plg
Gene Symbol
PLG
Additional Plasminogen Products
Product Documents for Human Plasminogen Antibody
Product Specific Notices for Human Plasminogen Antibody
For research use only
Loading...
Loading...
Loading...
Loading...