Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Plexin B1 Monoclonal Antibody (Catalog # MAB37491, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Applications for Human Plexin B1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin B1

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha-chain (aa 20-1305) and a 100 kDa TM beta-chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with neuropilins, MET, and EGF R2. Of three known splice variants, one shows a deletion of aa 689-871, a second shows a 53 aa substitution for aa 677‑2135, and a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 shares 90% and 97% aa sequence identity with dog and mouse Plexin B1, respectively.

Alternate Names

PLXN5, PLXNB1, Transmembrane Protein SEP

Entrez Gene IDs

5364 (Human); 235611 (Mouse); 316009 (Rat)

Gene Symbol

PLXNB1

UniProt

O43157

Additional Plexin B1 Products

Product Documents for Human Plexin B1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Plexin B1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Plexin B1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB37491

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Plexin B1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Plexin B1 Antibody

Detection of Plexin B1 in Jurkat Human Cell Line by Flow Cytometry.