Human Plexin B2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5329

R&D Systems, part of Bio-Techne
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MAB5329
MAB5329-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Functional Assay, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 537237

View all Plexin B2 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Plexin B2
Leu20-Arg1160
Accession # O15031

Specificity

Detects human Plexin B2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Plexin A4, B1, B3, C1, D1, or recombinant mouse Plexin A1, A2, or A3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Plexin B2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Plexin B2 under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB5329 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Plexin B2

Plexin B2 is a 240 kDa member of the B subfamily, plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes, and serves as a receptor for Semaphorin 4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178 aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20‑466) and three IPT repeats (aa 803‑1092). The ECD may be cleaved into two subunits, a 170 kDa alpha-chain (aa 20‑1164) and an 80 kDa TM beta‑chain that remain noncovalently‑linked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa 67‑638. Over aa 20‑1160, human Plexin B2 is 82% aa identical to mouse Plexin B2.

Alternate Names

MM1, PLEXB2, PLXNB2

Entrez Gene IDs

23654 (Human); 140570 (Mouse); 315217 (Rat)

Gene Symbol

PLXNB2

UniProt

O15031

Additional Plexin B2 Products

Product Documents for Human Plexin B2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Plexin B2 Antibody

For research use only

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