Human PLRP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7105
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PLRP1
Lys18-Cys467
Accession # P54315
Lys18-Cys467
Accession # P54315
Specificity
Detects human PLRP1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PLRP1 Antibody
Detection of Human PLRP1 by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7105) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLRP1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PLRP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human pancreas tissue
Sample: Human pancreas tissue
Reviewed Applications
Read 1 review rated 5 using AF7105 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLRP1
PNLIPRP1 (pancreatic lipase related protein 1; also PLRP1) is a secreted 50-55 kDa member of the lipase family, AB hydrolase superfamily of proteins. It is expressed by sublingual gland epithelium (in mouse) and pancreatic acinar cells, and is now suggested to act as a negative regulator of pancreatic lipase (PL) activity by competing with PL for colipase occupancy. Mature human PLRP1 is 450 amino acids (aa) in length (aa 18-467). It contains one PLAT domain (aa 356-467), and a Val196/Ala198 motif that inactivates its otherwise expected intrinsic lipase activity. There are two isoform variants. One shows a 76 aa substitution for aa 111-467, while another shows a seven aa substitution for aa 111-467. Mature human PLRP1 (aa 18-467) shares 83% aa identity with mature mouse PLRP1.
Long Name
Pancreatic Lipase-related Protein 1
Alternate Names
PNLIPRP1
Gene Symbol
PNLIPRP1
UniProt
Additional PLRP1 Products
Product Documents for Human PLRP1 Antibody
Product Specific Notices for Human PLRP1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...