Human Polycystin-1/PKD1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6270

R&D Systems, part of Bio-Techne
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AF6270
AF6270-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Polycystin-1/PKD1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Polycystin-1/PKD1
Gly4142-Thr4303
Accession # P98161

Specificity

Detects human Polycystin-1/PKD1 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Polycystin-1/PKD1 Antibody

Polycystin-1/PKD1 antibody in Human HUVEC by Immunocytochemistry (ICC).

Polycystin‑1/PKD1 in Human HUVEC.

Polycystin‑1/PKD1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Sheep Anti-Human Polycystin‑1/PKD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6270) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membranes, cytoplasm and endoplasmic reticula. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Polycystin-1/PKD1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Polycystin-1/PKD1

Polycystin-1 (also PKD1) is a 500-550 kDa member of the polycystin family of proteins. It is expressed in renal tubule primary cilia, and the membrane region that forms adherens junctions. Polycystin-1 binds to polycystin-2, promoting its insertion into the cell membrane, and regulating its calcium channel activity. In conjunction with polycystin-2, it detects fluid flow and converts this information into calcium signals. It also exists in the ER, where it negatively modulates polycystin-2 mediated calcium release. Mature human polycystin-1 is a 4280 amino acid (aa), 11 transmembrane glycoprotein. Its N-terminal extracellular region (aa 24-3074) is highly modular, and contains a C-type lecin domain, multiple Leu-rich repeats and PKD domains, one GPS region and a PLAT, REJ, WSC and LDLR domain. The cytoplasmic C-terminus (aa 4107-4303) contains a polycystin-2 coiled-coil interaction domain (aa 4220-4251). A percentage of polycystin-1 undergoes autoproteolysis after Leu3048, generating a presumed N-terminal fragment of ~ 350-400 kDa, and a membrane-bound C-terminal fragment of 150 kDa. Over aa 4141-4303, human polycystin-1 shares 71% aa identity with mouse polycystin-1.

Alternate Names

Pc-1, PKD1, Polycystin1, TRPP1

Entrez Gene IDs

5310 (Human); 18763 (Mouse)

Gene Symbol

PKD1

UniProt

P98161 (Human)

Additional Polycystin-1/PKD1 Products

Product Documents for Human Polycystin-1/PKD1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Polycystin-1/PKD1 Antibody

For research use only

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