Human Polypeptide GalNAc Transferase 4/GALNT4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7528
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Polypeptide GalNac Transferase 4/GALNT4
Ala36-Lys578
Accession # Q8N4A0
Ala36-Lys578
Accession # Q8N4A0
Specificity
Detects human Polypeptide GalNac Transferase 4/GALNT4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) GALNT1 and rhGALNT3 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Polypeptide GalNAc Transferase 4/GALNT4 Antibody
Detection of Human and Mouse Polypeptide GalNac Transferase 4/GALNT4 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Polypeptide GalNac Transferase 4/GALNT4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7528) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Polypeptide GalNac Transferase 4/GALNT4 at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Polypeptide GalNAc Transferase 4/GALNT4 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and mouse liver tissue
Sample: A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and mouse liver tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Polypeptide GalNAc Transferase 4/GALNT4
Long Name
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4
Alternate Names
GALNACT4
Gene Symbol
GALNT4
UniProt
Additional Polypeptide GalNAc Transferase 4/GALNT4 Products
Product Documents for Human Polypeptide GalNAc Transferase 4/GALNT4 Antibody
Product Specific Notices for Human Polypeptide GalNAc Transferase 4/GALNT4 Antibody
For research use only
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