Human PON1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5816
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PON1
Ala30-Leu355
Accession # P27169
Ala30-Leu355
Accession # P27169
Specificity
Detects human PON1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PON1 Antibody
Detection of Human PON1 by Western Blot.
Western blot shows lysates of human liver tissue . PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human PON1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5816) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, an enriched HDL/LDL fraction obtained from ultracentrifugation of human plasma was included. A specific band for PON1 was detected at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human PON1 by Simple WesternTM.
Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for PON1 at approximately 52 kDa (as indicated) using 5 µg/mL of Goat Anti-Human PON1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5816) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human PON1 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Western Blot
0.5 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF5816 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PON1
Long Name
Paraoxonase 1
Alternate Names
ESA, K-45
Gene Symbol
PON1
UniProt
Additional PON1 Products
Product Documents for Human PON1 Antibody
Product Specific Notices for Human PON1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...