Human PON2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4344
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PON2
Ala30-Leu354
Accession # Q15165
Ala30-Leu354
Accession # Q15165
Specificity
Detects endogenous human PON2 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human PON1 or PON3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PON2 Antibody
Detection of Human PON2 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human PON2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4344) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PON2 at approximately 42-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human PON2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PON2
The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/arylesterase 2 (PON2) is a 354 amino acid, 39 kDa protein that is widely expressed in a variety of tissues and may act as a cellular antioxidant, protecting cells from oxidative stress. PON2 has arylesterase and aryldialkylphosphatase activity (EC 3.1.1.2 and EC 3.1.8.1) and can hydrolyze a number of organophosphate substrates and aromatic carboxylic acid esters. PON2 is membrane-bound and has several potential glycosylation sites. Sequence polymorphisms in this gene may be associated with coronary heart disease and a number of phenotypes related to diabetes. PON2 is not associated with HDL but can prevent LDL lipid peroxidation and reverse the oxidation of mildly oxidized LDL. Alternatively spliced transcript variants encoding different isoforms have been described.
Long Name
Paraoxonase 2
Alternate Names
A-esterase 2, Serum Aryldialkylphosphatase 2
Gene Symbol
PON2
UniProt
Additional PON2 Products
Product Documents for Human PON2 Antibody
Product Specific Notices for Human PON2 Antibody
For research use only
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