Human/Porcine/Equine Integrin beta1/CD29 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB17783
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Porcine, Equine
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 419127
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta1/CD29
Gln21-Asp728 (predicted)
Accession # P05556
Gln21-Asp728 (predicted)
Accession # P05556
Specificity
Detects human Integrin beta1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta3, beta4, beta5, beta8, recombinant mouse Integrin beta1, beta2, beta6, or beta7 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Porcine/Equine Integrin beta1/CD29 Antibody
Detection of Integrin beta1/CD29 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (MAB004), open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).
Detection of Integrin beta1/CD29 in Porcine Mesenchymal Stem Cells by Flow Cytometry.
Porcine mesenchymal stem cells were stained with Mouse Anti-Human/Porcine/Equine Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Detection of Integrin beta1/CD29 in Equine PBMCs by Flow Cytometry.
Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Porcine/Equine Integrin beta1/CD29 Monoclonal Antibody (Catalog # MAB17783, filled histogram) or isotype control antibody (MAB004, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).Applications for Human/Porcine/Equine Integrin beta1/CD29 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs), porcine mesenchymal stem cells, and equine peripheral blood mononuclear cells (PBMCs)
Sample: Human peripheral blood mononuclear cells (PBMCs), porcine mesenchymal stem cells, and equine peripheral blood mononuclear cells (PBMCs)
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MG-63 human osteosarcoma cell line and porcine mesenchymal stem cells
Sample: Immersion fixed MG-63 human osteosarcoma cell line and porcine mesenchymal stem cells
Western Blot
1 µg/mL
Sample: Recombinant Human Integrin beta1/CD29 under non-reducing conditions only
Sample: Recombinant Human Integrin beta1/CD29 under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 1/CD29
Alternate Names
CD29, ITGB1
Gene Symbol
ITGB1
UniProt
Additional Integrin beta 1/CD29 Products
Product Documents for Human/Porcine/Equine Integrin beta1/CD29 Antibody
Product Specific Notices for Human/Porcine/Equine Integrin beta1/CD29 Antibody
For research use only
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