Human POT1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5299
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human POT1
Met159-Pro316
Accession # Q9NUX5
Met159-Pro316
Accession # Q9NUX5
Specificity
Detects endogenous human POT1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human POT1 Antibody
Detection of Human POT1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human POT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5299) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for POT1 at approximately 71 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human POT1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and 293T human embryonic kidney cell line
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and 293T human embryonic kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: POT1
POT1 (Protection of telomeres protein 1; also POTE1) is a nuclear, 71 kDa member of the telombin family of proteins. It is ubiquitously expressed, and binds single stranded DNA, TRF2, and TPP1 at the end of chromosomes. This stabilizes chromosome ends and protects against unwarranted activation of DNA damage programs. Human POT1 is 634 amino acids (aa) in length. It contains two N-terminal DNA binding OB regions (aa 1‑300) and a C-terminal TPP-1 dimerization domain (aa 301‑634). There are multiple isoform variants. There is a potential alternate start site at Met132, plus a three aa substitution for aa 43‑634, a 24 aa substitution for aa 317‑634, a three aa substitution for aa 457‑634, and a 16 aa substitution for aa 503‑634. Over aa 159‑316, human POT1 shares 71% aa identity with mouse POT1.
Long Name
Protection of Telomeres Protein 1
Alternate Names
DKFZp586D211, HPOT1, POT1 protection of telomeres 1 homolog, POT1-like telomere end-binding protein, protection of telomeres 1 homolog (S. pombe), protection of telomeres protein 1
Gene Symbol
POT1
UniProt
Additional POT1 Products
Product Documents for Human POT1 Antibody
Product Specific Notices for Human POT1 Antibody
For research use only
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