Human PRDM14 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6175
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PRDM14
Ala2-Asp201
Accession # Q9GZV8
Ala2-Asp201
Accession # Q9GZV8
Specificity
Detects human PRDM14 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PRDM14 Antibody
Detection of Human PRDM14 by Western Blot.
Western blot shows lysates of BG01V human embryonic stem cells (undifferentiated) and NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Human PRDM14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6175) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRDM14 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PRDM14 in BG01V Human Embryonic Stem Cells.
PRDM14 was detected in immersion fixed BG01V human embryonic stem cells using Sheep Anti-Human PRDM14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6175) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, upper panel; Catalog # NL012) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human PRDM14 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion-fixed BG01V human embryonic stem cells
Sample: Immersion-fixed BG01V human embryonic stem cells
Western Blot
0.1 µg/mL
Sample: BG01V human embryonic stem cells (undifferentiated) and NTera‑2 human testicular embryonic carcinoma cell line
Sample: BG01V human embryonic stem cells (undifferentiated) and NTera‑2 human testicular embryonic carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRDM14
PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266-367) and six zinc finger repeats (aa 400‑568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is up‑regulated in breast cancer and lymphoblastic leukemia. Within aa 1-201, human and mouse PRDM14 share 46% aa sequence identity.
Long Name
PR Domain Containing 14
Alternate Names
MGC59730, PFM11, PR domain containing 14, PR domain zinc finger protein 14, PR domain-containing protein 14, PR-domain zinc finger protein 14
Gene Symbol
PRDM14
UniProt
Additional PRDM14 Products
Product Documents for Human PRDM14 Antibody
Product Specific Notices for Human PRDM14 Antibody
For research use only
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