Human Pro BDNF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB31752
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 584431
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Pro-BDNF
Met1-Arg247
Accession # P23560
Met1-Arg247
Accession # P23560
Specificity
Detects human Pro-BDNF in direct ELISAs and sandwich immunoassays.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Pro BDNF Antibody
Human Pro-BDNF ELISA Standard Curve.
Recombinant Human Pro-BDNF protein was serially diluted 2-fold and captured by Mouse Anti-Human Pro-BDNF Monoclonal Antibody (Catalog # MAB31752) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB848) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Pro BDNF Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB848).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pro-BDNF DuoSet ELISA Kit (Catalog # DY3175) for convenient development of a sandwich ELISA.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BDNF
BDNF is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of neuronal subpopulations in the central and peripheral nervous systems. BDNF functions through interactions with the TrkB receptor tyrosine kinase and the low affinity neurotrophin receptor, p75 (NTR). The human BDNF cDNA encodes 247 amino acids (aa). Cleavage of an 18 aa signal sequence produces an approximately 35 kDa Pro-BDNF form. The N-terminal pro region of BDNF is removed by tPA and furin to release biologically active, 14 kDa BDNF. The propeptides of human and mouse BDNF share 93% aa sequence identity.
Long Name
Brain-derived Neurotrophic Factor
Alternate Names
Abrineurin, ANON2, BULN2, Neurotrophin
Gene Symbol
BDNF
UniProt
Additional BDNF Products
Product Documents for Human Pro BDNF Antibody
Product Specific Notices for Human Pro BDNF Antibody
For research use only
Loading...
Loading...
Loading...
Loading...