Human Pro-Corticoliberin/Pro-CRH Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5805
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Pro‑Corticoliberin/Pro‑CRH
Leu25-Lys196
Accession # P06850
Leu25-Lys196
Accession # P06850
Specificity
Detects human Pro‑Corticoliberin/Pro‑CRH in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Pro-Corticoliberin/Pro-CRH Antibody
Detection of Human Pro-Corticoliberin/Pro-CRH by Western Blot.
Western blot shows lysates of human cerebellum and hypothalamus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Corticoliberin/Pro-CRH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5805) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-Corticoliberin/Pro-CRH at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human Pro-Corticoliberin/Pro-CRH Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human cerebellum and hypothalamus tissue
Sample: Human cerebellum and hypothalamus tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pro-Corticoliberin/Pro-CRH
Long Name
Pro-Corticotropin-releasing Hormone
Alternate Names
CRF, CRH, ProCorticoliberin
Gene Symbol
CRH
UniProt
Additional Pro-Corticoliberin/Pro-CRH Products
Product Documents for Human Pro-Corticoliberin/Pro-CRH Antibody
Product Specific Notices for Human Pro-Corticoliberin/Pro-CRH Antibody
For research use only
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