Human Pro‑MMP‑7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB907
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 6A4
Product Specifications
Immunogen
Human MMP-7 synthetic peptide
AEYSLFPNSPKWTSKV, Ala93-Val108
Accession # P09237
AEYSLFPNSPKWTSKV, Ala93-Val108
Accession # P09237
Specificity
Detects recombinant human (rh) Pro-MMP-7 (aa 18-267) in Western blots. In Western blots, no cross-reactivity with rhMMP-1, rhMMP-2, rhMMP-9 or mature rhMMP-7 (aa 95-267) is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Pro‑MMP‑7 Antibody
MMP-7 in Human Pancreatic Cancer Tissue.
MMP-7 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Human Pro-MMP-7 Monoclonal Antibody (Catalog # MAB907) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Pro‑MMP‑7 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (neuroblastoma) and human pancreatic cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human brain (neuroblastoma) and human pancreatic cancer tissue
Western Blot
1 µg/mL
Sample: Recombinant Human MMP-7 Western Blot Standard (Catalog # WBC016)
Sample: Recombinant Human MMP-7 Western Blot Standard (Catalog # WBC016)
Reviewed Applications
Read 1 review rated 5 using MAB907 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-7
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal alpha-defensin activation in innate host defense, releases tumor necrosis factor-alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.
Long Name
Matrix Metalloproteinase 7
Alternate Names
Matrilysin, MMP7
Gene Symbol
MMP7
UniProt
Additional MMP-7 Products
Product Documents for Human Pro‑MMP‑7 Antibody
Product Specific Notices for Human Pro‑MMP‑7 Antibody
For research use only
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