Human Profilin 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7779
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 816536
Product Specifications
Immunogen
E. coli-derived recombinant human Profilin 1
Met1-Tyr140
Accession # P07737
Met1-Tyr140
Accession # P07737
Specificity
Detects human Profilin 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity
with recombinant human Profilin 2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Profilin 1 Antibody
Detection of Human and Mouse Profilin 1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Profilin 1 Monoclonal Antibody (Catalog # MAB7779) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Profilin 1 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Profilin 1 by Simple WesternTM.
Simple Western shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Profilin 1 at approximately 18 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Profilin 1 Monoclonal Antibody (Catalog # MAB7779). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.Applications for Human Profilin 1 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line and NIH‑3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Profilin 1
Profilin 1 (gene name PFN1) is a ubiquitous, cytoplasmic, 140 amino acid, 12-15 kDa actin monomer-binding protein. It facilitates adenine nucleotide exchange, supplying ATP for actin filament polymerization. It is phosphorylated and acetylated, and binds proline-rich sequences. Human Profilin 1 shares 96% amino acid sequence identity with mouse and rat Profilin 1.
Alternate Names
PFN1, PROF1, Profilin I
Gene Symbol
PFN1
UniProt
Additional Profilin 1 Products
Product Documents for Human Profilin 1 Antibody
Product Specific Notices for Human Profilin 1 Antibody
For research use only
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