Human Prostaglandin I2 Synthase Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7788
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Prostaglandin I2 Synthase
Arg21-Pro500
Accession # Q16647
Arg21-Pro500
Accession # Q16647
Specificity
Detects human Prostaglandin I2 Synthase in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Prostaglandin I2 Synthase Antibody
Detection of Human Prostaglandin I2 Synthase by Western Blot.
Western blot shows lysates of SK-OV-3 human ovarian adenocarcinoma cell line, human ovary tissue, human prostate tissue, and human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Prostaglandin I2 Synthase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7788) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Prostaglandin I2 Synthase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Prostaglandin I2 Synthase Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: SK‑OV‑3 human ovarian adenocarcinoma cell line, human ovary tissue, human prostate tissue, and human heart tissue
Sample: SK‑OV‑3 human ovarian adenocarcinoma cell line, human ovary tissue, human prostate tissue, and human heart tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prostaglandin I2 Synthase
Alternate Names
CYP8, CYP8A1, PGIS, Prostacyclin Synthase, PTGI
Gene Symbol
PTGIS
UniProt
Additional Prostaglandin I2 Synthase Products
Product Documents for Human Prostaglandin I2 Synthase Antibody
Product Specific Notices for Human Prostaglandin I2 Synthase Antibody
For research use only
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