Human Protocadherin-15 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6729
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 740523
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Protocadherin-15
Tyr28-Ala1376
Accession # Q96QU1
Tyr28-Ala1376
Accession # Q96QU1
Specificity
Detects human Protocadherin-15 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Protocadherin-15 Antibody
Protocadherin‑15 in YT Human Cell Line.
Protocadherin-15 was detected in immersion fixed YT human leukemia natural killer-like cell line using Mouse Anti-Human Protocadherin-15 Monoclonal Antibody (Catalog # MAB6729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Protocadherin-15 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed YT human leukemia natural killer‑like cell line
Sample: Immersion fixed YT human leukemia natural killer‑like cell line
Reviewed Applications
Read 3 reviews rated 4.3 using MAB6729 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protocadherin-15
Alternate Names
CDHR15, DFNB23, PCDH15, Protocadherin15, USH1F
Gene Symbol
PCDH15
UniProt
Additional Protocadherin-15 Products
Product Documents for Human Protocadherin-15 Antibody
Product Specific Notices for Human Protocadherin-15 Antibody
For research use only
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