Human Protocadherin-8 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5434
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Protocadherin-8
Lys30-Pro749
Accession # O95206
Lys30-Pro749
Accession # O95206
Specificity
Detects human Protocadherin-8 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human Protocadherin-10 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Protocadherin-8 Antibody
Detection of Human Protocadherin‑8 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Protocadherin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5434) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Protocadherin-8 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Protocadherin‑8 in MCF‑7 Human Cell Line.
Protocadherin-8 was detected in immersion fixed MCF-7 human breast cancer cell line using Human Protocadherin-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5434) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Protocadherin-8 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line
Sample: HT‑29 human colon adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protocadherin-8
Protocadherin 8 (PCDH-8, Arcadlin) is a 110-115 kDa group F member of the protocadherin family of molecules. It is expressed in breast epithelium, the synaptic membranes of neurons, and presumably forms Ca++-dependent homophilic complexes. In breast, it is believed to contribute to a stable epithelial architecture, while in brain, it impacts LTP. Mature human PCDH-8 is a type I transmembrane protein that is 1041 amino acids (aa) in length. It contains a 720aa extracellular domain (ECD) (aa 30-749) plus a 300 aa cytoplasmic region. There are six cadherin domains in the ECD (aa 30-723). Multiple splice variants exist. Two show deletions of aa 134-607 and 781-877, respectively, while a third shows a six aa substitution for aa 365-527. Over aa 30-689, human PCDH-8 shares 93% aa identity with mouse PCDH-8.
Alternate Names
Arcadlin, PAPC, PCDH8, Protocadherin8
Gene Symbol
PCDH8
UniProt
Additional Protocadherin-8 Products
Product Documents for Human Protocadherin-8 Antibody
Product Specific Notices for Human Protocadherin-8 Antibody
For research use only
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