Human Protogenin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6795
Key Product Details
Species Reactivity
Validated:
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 814403
Product Summary for Human Protogenin Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Protogenin
Phe36-Ala952
Accession # Q2VWP7
Phe36-Ala952
Accession # Q2VWP7
Specificity
Detects human Protogenin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Protogenin Antibody
Adhesion Induced by Protogenin and Neutralization by Human Protogenin Antibody.
Recombinant Human Protogenin (Catalog # 6795-PR) supports the adhesion in the P19 mouse embryonal carcinoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Protogenin (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Protogenin Monoclonal Antibody (Catalog # MAB6795). The ND50 is typically 0.4-2.4 µg/mL.Applications for Human Protogenin Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Protogenin-induced adhesion in the P19 mouse embryonal carcinoma cell line. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 10 µg/mL Recombinant Human Protogenin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protogenin
(aa 36-952) and a 180 aa cytoplasmic region. The ECD possesses four consecutive Ig-like domains (aa 36-411) followed by five FN type III domains (aa 419-914). There is one potential splice variant that shows a 49 aa substitution for Ala325 coupled to a deletion of aa 516-541. The ECD of human PRTG shares 93% aa identity with the ECD of mouse PRTG.
Alternate Names
IGDCC5, PRTG, RPTG, Shen-Dan
Gene Symbol
PRTG
UniProt
Additional Protogenin Products
Product Documents for Human Protogenin Antibody
Product Specific Notices for Human Protogenin Antibody
For research use only
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