Human Protogenin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6795
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Protegenin
Phe36-Ala952
Accession # Q2VWP7
Phe36-Ala952
Accession # Q2VWP7
Specificity
Detects human Protegenin in direct ELISAs. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Protegenin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human Protogenin Antibody
Protogenin in Mouse Embryo.
Protogenin was detected in immersion fixed frozen sections of mouse embryo (E9.5) using Sheep Anti-Human Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6795) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012) and counterstained with DAPI (blue). Specific staining was localized to nuclei in the neural tube. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human Protogenin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E9.5)
Sample: Immersion fixed frozen sections of mouse embryo (E9.5)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protogenin
PRTG (Protogenin; also Protein Shen-Dan) is a 175-180 kDa transmembrane (TM) member of the DEAL (DCC et al) family of proteins. It is expressed in the embryo in the period before E10.5 in mouse (day 28 in human), and appears in a widespread distribution. Through homophilic intercellular interaction, it regulates initial epiblast cell migration/ingression, thus impacting somite organization. Via its actions as a neural tube epithelial cell receptor, it binds soluble 60 kDa ERdj3, resulting in a suppression of neuron differentiation. Mature human PRTG is an 1115 amino acid (aa) type I TM glycoprotein. It contains a 917 aa extracellular domain (ECD) (aa 36‑952) and a 180 aa cytoplasmic region. The ECD possesses four consecutive Ig-like domains (aa 36‑411) followed by five FN type III domains (aa 419-914). There is one potential splice variant that shows a 49 aa substitution for Ala325 coupled to a deletion of aa 516-541. The ECD of human PRTG shares 93% aa identity with the ECD of mouse PRTG.
Alternate Names
IGDCC5, PRTG, RPTG, Shen-Dan
Gene Symbol
PRTG
UniProt
Additional Protogenin Products
Product Documents for Human Protogenin Antibody
Product Specific Notices for Human Protogenin Antibody
For research use only
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