Human PTGER1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB100081

R&D Systems, part of Bio-Techne
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MAB100081-100
MAB100081-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1014918

View all PTGER1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human PTGER1
Accession # P34995

Specificity

Detects human PTGER1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PTGER1 Antibody

PTGER1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

PTGER1 in Human Breast Cancer Tissue.

PTGER1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PTGER1 Monoclonal Antibody (Catalog # MAB100081) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human PTGER1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB100081 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTGER1

PTGER1 is a one of four EP1 receptors for PGE2 which is mediated by G(q) proteins.  PTGER1 may play a role as an important modulator of renal functions.  It has also been detected in mast cells, pulmonary veins, keratinocytes, myometrium and colon smooth muscle. EP1 expression has also frequently been seen in human breast cancers.  Nuclear expression of EP1 correlates with good prognosis markers like node negative status and PR expression. Animal model studies have implicated EP1 in various physiological and pathological responses however expression in humans appears to be more limited. EP1 receptor antagonists have been studied clinically primarily to treat hyperalgesia.

Long Name

Prostaglandin E2 Receptor 1, Subtype EP1

Alternate Names

EP1

Entrez Gene IDs

5731 (Human); 19216 (Mouse); 25637 (Rat)

Gene Symbol

PTGER1

UniProt

P34995 (Human)

Additional PTGER1 Products

Product Documents for Human PTGER1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PTGER1 Antibody

For research use only

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