Human PTPN13/PTPL1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3577
Discontinued Product
MAB3577 has been discontinued.
An alternative/replacement product is available:
AF3577. View all PTPN13/PTPL1 products.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 359313
Product Specifications
Immunogen
E. coli-derived recombinant human PTPN13/PTPL1
His2-Arg500
Accession # Q12923
His2-Arg500
Accession # Q12923
Specificity
Detects endogenous human PTPN13/PTPL1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PTPN13/PTPL1 Antibody
Detection of Human PTPN13/PTPL1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN13/PTPL1 Monoclonal Antibody (Catalog # MAB3577) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPN13/PTPL1 at approximately 260 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Human PTPN13/PTPL1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTPN13/PTPL1
Protein Tyrosine Phosphatase, Non-receptor Type 13, also called PTPN13, PTPL1, FAP-1, hPTP1E, and PTP-BAS, is a 260 kDa intracellular protein that removes phosphates from tyrosine residues. Genetic aberrations in PTPN13 have been reported in a wide variety of cancers, including bone, colon, hepatocellular, and germ cell line. Elevated levels of PTPN13 in cancer cell lines have also been correlated with resistance to Fas-induced apoptosis, possibly due to prevention of CD95 reaching the cell surface.
Long Name
Protein Tyrosine Phosphatase, Non-receptor Type 13
Alternate Names
EAP-1, FAP-1, PNP1, PTP-BAS, PTP-BL, PTP1E, PTPL1, PTPLE
Gene Symbol
PTPN13
UniProt
Additional PTPN13/PTPL1 Products
Product Documents for Human PTPN13/PTPL1 Antibody
Product Specific Notices for Human PTPN13/PTPL1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...