Human PTPN14 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4458
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 448701
Product Specifications
Immunogen
E. coli-derived recombinant human PTPN14
Leu329-Leu900
Accession # Q15678.2
Leu329-Leu900
Accession # Q15678.2
Specificity
Detects endogenous human PTPN14 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PTPN13 or rhPTPN22.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PTPN14 Antibody
Detection of Human PTPN14 by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPN14 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PTPN14 in Human Placenta.
PTPN14 was detected in immersion fixed paraffin-embedded sections of human placenta using Human PTPN14 Monoclonal Antibody (Catalog # MAB4458) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PTPN14 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded human placenta
Sample: Immersion fixed paraffin-embedded human placenta
Western Blot
1 µg/mL
Sample: DU145 human prostate carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line
Sample: DU145 human prostate carcinoma cell line, HT-29 human colon adenocarcinoma cell line, and HT-29 human colon adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4 using MAB4458 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTPN14
Protein Tyrosine Phosphatase, Non-receptor type 14 (PTPN14), also called Phosphatase with Ezrin Domain (PEZ) and PTP36, is a 135 kDa cytosolic protein that dephosphorylates tyrosine residues in proteins. PTPN14’s Ezrin domain is homologous to erythrocyte Band 4.1 protein, targeting the phosphatase to cytoskeletal elements such as actin. PTPN14 associates with beta-catenin in adhesion zones in cells that are touching, but is nuclear in isolated, proliferating cells. Loss-of-function mutations in PTPN14 are found in 26% of colorectal cancers, suggesting it is a tumor suppressor.
Long Name
Protein Tyrosine Phosphatase Non-receptor Type 14
Alternate Names
PEZ
Gene Symbol
PTPN14
UniProt
Additional PTPN14 Products
Product Documents for Human PTPN14 Antibody
Product Specific Notices for Human PTPN14 Antibody
For research use only
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