Human Pygopus-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3616
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Pygopus-2
Met160-Glu336
Accession # Q9BRQ0
Met160-Glu336
Accession # Q9BRQ0
Specificity
Detects human Pygopus-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human Pygopus‑1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Pygopus-2 Antibody
Detection of Human Pygopus-2 by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pygopus-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Pygopus-2 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Pygopus‑2 in Human Ovary and Human Ovarian Cancer Tissue.
Pygopus-2 was detected in immersion fixed paraffin-embedded sections of normal human ovary (left panel) and ovarian cancer tissue (right panel) using Goat Anti-Human Pygopus-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Pygopus-2 Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections human ovary and human ovarian cancer tissue
Sample: Immersion fixed paraffin-embedded sections human ovary and human ovarian cancer tissue
Western Blot
1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: SH‑SY5Y human neuroblastoma cell line and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pygopus-2
Alternate Names
PYGO2, Pygopus2
Gene Symbol
PYGO2
UniProt
Additional Pygopus-2 Products
Product Documents for Human Pygopus-2 Antibody
Product Specific Notices for Human Pygopus-2 Antibody
For research use only
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