Human Rab13 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8305
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 863028
Product Specifications
Immunogen
E. coli-derived recombinant human Rab13
Lys94-Thr191
Accession # P51153
Lys94-Thr191
Accession # P51153
Specificity
Detects human Rab13 in direct ELISA and Western Blot.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Rab13 Antibody
Detection of Human Rab13 by Western Blot.
Western blot shows lysates of human lung tissue and human aorta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Rab13 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Rab13 in Caco‑2 Human Cell Line.
Rab13 was detected in immersion fixed Caco-2 human colorectal adenocarcinoma cell line using Mouse Anti-Human Rab13 Monoclonal Antibody (Catalog # MAB8305) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Rab13 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed Caco‑2 human colorectal adenocarcinoma cell line
Sample: Immersion fixed Caco‑2 human colorectal adenocarcinoma cell line
Western Blot
2 µg/mL
Sample: Human lung tissue and human aorta tissue
Sample: Human lung tissue and human aorta tissue
Reviewed Applications
Read 2 reviews rated 5 using MAB8305 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rab13
Long Name
RAs Genes from Brain Protein 13
Alternate Names
Cell growth-inhibiting gene 4 protein, growth-inhibiting gene 4 protein, RAB13, member RAS oncogene family, RAS-associated protein RAB13, ras-related protein Rab-13
Gene Symbol
RAB13
UniProt
Additional Rab13 Products
Product Documents for Human Rab13 Antibody
Product Specific Notices for Human Rab13 Antibody
For research use only
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