Human RAB25 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6605
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RAB25
Met1-Ala195
Accession # P57735
Met1-Ala195
Accession # P57735
Specificity
Detects human RAB25 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human RAB25 Antibody
Detection of Human RAB25 by Western Blot.
Western blot shows lysates of HT-29 human colon adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and SK-BR-3 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human RAB25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for RAB25 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human RAB25 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and SK‑BR‑3 human breast cancer cell line
Sample: HT‑29 human colon adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and SK‑BR‑3 human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rab25
Ras-related protein Rab25, also known as CATX-8, is a 23 kDa (unglycosylated) member of the small GTPase superfamily and Rab family of proteins. Human Rab25 is 213 amino acids (aa) in length. The mature chain runs from aa 1-210 with residues 211-213 constituting a propeptide. Residues 10-174 are noted to be Rab11-like. Rab11a, Rab11b, and Rab25 are closely related Rab proteins that are differentially expressed. Human Rab25 shares 96% aa sequence identity with mouse Rab25. Functionally, Rab25 is involved in the regulation of cell survival. Researchers have shown that Rab25 promotes invasive migration of cells and increases the rates and aggressiveness of breast and ovarian cancers. It is expressed in breast and ovarian tissues.
Long Name
RAs Genes from Brain Protein 25
Alternate Names
CATX8, RAB11C
Gene Symbol
RAB25
UniProt
Additional Rab25 Products
Product Documents for Human RAB25 Antibody
Product Specific Notices for Human RAB25 Antibody
For research use only
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