Human RalB Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3920
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 399417
Product Specifications
Immunogen
E. coli-derived recombinant human RalB
Met1-Leu206
Accession # P11234
Met1-Leu206
Accession # P11234
Specificity
Detects human, mouse, and rat RalB in Western blots. In Western blots, no cross-reactivity with recombinant human RalA is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human RalB Antibody
Detection of Human RalB by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human RalB Monoclonal Antibody (Catalog # MAB3920) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human RalA and RalB (10 ng/lane) were included. A specific band for RalB was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human RalB Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: U937 human histiocytic lymphoma cell line and Y3-Ag rat myeloid cell line
Sample: U937 human histiocytic lymphoma cell line and Y3-Ag rat myeloid cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RalB
The Ras-like proteins RalA and RalB share 85% identity and constitute a grouping within the Ras superfamily of small GTPases. Like other GTPases, Ral proteins transduce signals by cycling between an active GTP-bound and an inactive GDP-bound state. Ral functions as an effector of Ras-mediated signaling, and has been implicated in the regulation of vesicle trafficking and cell morphology.
Long Name
Ras-like Protein B
Alternate Names
GTP binding protein, RAS-like protein B, ras-related protein Ral-B, v-ral simian leukemia viral oncogene homolog B (ras related; GTP bindingprotein)
Gene Symbol
RALB
UniProt
Additional RalB Products
Product Documents for Human RalB Antibody
Product Specific Notices for Human RalB Antibody
For research use only
Loading...
Loading...
Loading...
Loading...