Human RAMP3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4875
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RAMP3
Arg24-Val118
Accession # O60896
Arg24-Val118
Accession # O60896
Specificity
Detects human RAMP3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) RAMP1, rhRAMP2, and recombinant mouse RAMP2 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human RAMP3 Antibody
Detection of Human RAMP3 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RAMP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4875) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP3 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human RAMP3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and human brain tissue
Sample: Raji human Burkitt's lymphoma cell line and human brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RAMP3
RAMP3 (receptor activity modifying protein 3) is a 20-26 kDa member of the RAMP family of proteins. It is expressed on multiple cell types in conjunction with CRLR and NHERF-1, thereby generating a receptor complex for adrenomedullin (AM). Mature human RAMP3 is a 125 amino acid (aa) type I transmembrane glycoprotein that contains a 95 aa extracellular domain (ECD) (aa 24-118) and a 10 aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa 59-65 are critical for AM binding. There is one potential splice variant that shows a 49 aa substitution for the first 19 aa of the signal sequence. The human ECD is 88% aa identical to the mouse ECD. Members of RAMP family of proteins are known to form complexes with apparent molecular weights of 25 to 50 kDa that are resistant to denaturing and reducing agents.
Long Name
Receptor Activity Modifying Protein 3
Alternate Names
calcitonin receptor-like receptor activity modifying protein 3, Calcitonin-receptor-like receptor activity-modifying protein 3, CRLR activity-modifying protein 3, receptor (calcitonin) activity modifying protein 3, receptor (G protein-coupled) activity modifying protein 3, receptor activity modifying protein 3, receptor activity-modifying protein 3, receptor-activity-modifying protein 3
Gene Symbol
RAMP3
UniProt
Additional RAMP3 Products
Product Documents for Human RAMP3 Antibody
Product Specific Notices for Human RAMP3 Antibody
For research use only
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