Human RanGAP1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF7834

R&D Systems, part of Bio-Techne
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AF7834
AF7834-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all RanGAP1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human RanGAP1
Asn418-Val587
Accession # P46060

Specificity

Detects human RanGAP1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human RanGAP1 Antibody

Detection of Human RanGAP1 antibody by Western Blot.

Detection of Human RanGAP1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NTera-2 human testicular embryonic carcinoma cell line, IMR-32 human neuroblastoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human RanGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7834) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RanGAP1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human RanGAP1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NTera‑2 human testicular embryonic carcinoma cell line, IMR‑32 human neuroblastoma cell line, and HEK293T human embryonic kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RanGAP1

RANGAP-1 (RAN [RAs-related Nuclear protein] GTPase-Activating Protein 1; also Fug1) is a 82-88 kDa member of the RNA1 family of molecules. It is widely expressed, and serves as a regulator of the nuclear regulatory protein RAN. RAN, when bound to GTP, is active, and is involved in the onset and termination of mitosis, plus the import and export of protein into the nucleus. In the cytosol, RAN:GTP is complexed to an Importin:cargo complex. RANGAP-1 converts the RAN‑associated GTP into GDP, and this induces dissociation of the complex into RAN:GDP, Importin and cargo. The cargo stays in the cytosol, while both RAN:GDP and Importin now enter the nucleus. Here, RAN:GDP is converted into RAN:GTP by RCC1, where it binds to a newly formed Importin:cargo complex, and is exported out of the nucleus, completing a cycle. While in the cytoplasm, RANGAP-1 is phosphorylated and stabilized through SUMOlyation and an interaction with Ubc9 and RanBP2. Human RANGAP-1 is 587 amino acids (aa) in length. It contains six LRRs (aa 48-343) plus two utilized acetylation sites and five phosphorylated Ser/Thr, and there is one SUMOylation motif between aa 523-526. There are three potential splice forms. One contains a single Ser insertion after Gly330, while a second contains an alternative start site 55 aa upstream of the standard site. A third isoform possesses that same upstream start site coupled to a 25 aa substitution for aa 525-587. Over aa 418-587, human RANGAP-1 shares 82% aa sequence identity with mouse RANGAP-1.

Long Name

Ran GTPase Activating Protein 1

Alternate Names

Fug1

Entrez Gene IDs

5905 (Human); 19387 (Mouse); 362965 (Rat)

Gene Symbol

RANGAP1

UniProt

P46060 (Human)

Additional RanGAP1 Products

Product Documents for Human RanGAP1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human RanGAP1 Antibody

For research use only

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