Human RARRES3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6139
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human RARRES3
Asp12-Lys134
Accession # Q9UL19
Asp12-Lys134
Accession # Q9UL19
Specificity
Detects human RARRES3 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) RARRES1 and rhLatexin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human RARRES3 Antibody
RARRES3 in Raji Human Cell Line.
RARRES3 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human RARRES3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human RARRES3 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Sample: Immersion fixed Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RARRES3
RARRES3 (Retinoic acid receptor responder 3; also Tig3) is an 18-20 kDa member of the lecithin retinol acyltransferase family of molecules. It is widely expressed, particularly in keratinocytes, and is considered to have multiple functions. These include phospholipase A activity and an ability to both activate type I transglutaminase, and inhibit Ras activation Human RARRES3 is 164 amino acids (aa) in length. It contains a catalytic residue at Cys113, and a hydrophobic membrane-anchoring domain between aa 134-164. Within the cell, RARRES3 appears to be equally distributed between membrane and soluble fractions. RARRES3 is also reported to form higher order multimers. One potential isoform exists that shows a 26 aa substitution for aa 131-164. No meaningful structural ortholog of RARRES3 exists in rodent.
Long Name
Retinoic Acid Receptor Responder [Tazarotene-induced] 3
Alternate Names
HRASLS4, TIG3
Entrez Gene IDs
5920 (Human)
Gene Symbol
PLAAT4
UniProt
Additional RARRES3 Products
Product Documents for Human RARRES3 Antibody
Product Specific Notices for Human RARRES3 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...