Human Ras Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3429
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 342404
Product Specifications
Immunogen
E. coli-derived recombinant human K-Ras2B
Met1-Met188 (Gln61His)
Accession # P01116-2
Met1-Met188 (Gln61His)
Accession # P01116-2
Specificity
Detects human Ras (H-Ras, N-Ras, and K-Ras) in Western blots. Does not detect more distantly related Ras superfamily members, including R-Ras, Ral, Rap, and Rheb.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human Ras Antibody
Detection of Human Ras by Western Blot.
Western blot shows lysates of K562 human chronic myelo-genous leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Human Ras Monoclonal Antibody (Catalog # MAB3429) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional refer-ence, recombinant human H-Ras, N Ras, K-Ras2A and 2B, R-Ras2 and 3, Rap2A and 2B, RalA and B, and Rheb (5 ng/lane) were included. A specific band for Ras was detected at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human Ras Antibody
Application
Recommended Usage
Western Blot
5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Ramos human Burkitt's lymphoma cell line
Sample: K562 human chronic myelogenous leukemia cell line and Ramos human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ras
The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.
Long Name
v-Ki-ras2 Kirsten Rat Sarcoma Viral Oncogene Jomolog
Alternate Names
c-K-ras, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, Ki-Ras, KRAS, KRAS1, KRAS2, Oncogene Ras, RASK2
Gene Symbol
KRAS
UniProt
Additional Ras Products
Product Documents for Human Ras Antibody
Product Specific Notices for Human Ras Antibody
For research use only
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