Human/Rat Annexin V Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB399
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Rat
Applications
Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 39103
Product Specifications
Immunogen
E. coli-derived recombinant human Annexin V
Met1-Asp320
Accession # P08758
Met1-Asp320
Accession # P08758
Specificity
Detects human Annexin V in direct ELISAs and Western blots. Detects rat Annexin V in Simple WesternsTM.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Rat Annexin V Antibody
Detection of Human Annexin V by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Annexin V Monoclonal Antibody (Catalog # MAB399) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Annexin V at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Annexin V by Simple WesternTM.
Simple Western lane view shows lysates of human lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Annexin V at approximately 39 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Rat Annexin V Monoclonal Antibody (Catalog # MAB399) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human Annexin V Specificity by Using Knockout Cell Line.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Annexin V knockout HEK293T cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Rat Annexin V Monoclonal Antibody (Catalog # MAB399) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Annexin V at approximately 32 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Rat Annexin V Antibody
Application
Recommended Usage
Knockout Validated
Annexin V
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
Annexin V knockout HEK293T cell line.
Simple Western
2.5 µg/mL
Sample: Human lung tissue and rat lung tissue
Sample: Human lung tissue and rat lung tissue
Western Blot
0.25 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Annexin V
Additional Annexin V Products
Product Documents for Human/Rat Annexin V Antibody
Product Specific Notices for Human/Rat Annexin V Antibody
For research use only
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