Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3960
Key Product Details
Species Reactivity
Human, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2
Lys29-Cys397
Accession # Q9NY97
Lys29-Cys397
Accession # Q9NY97
Specificity
Detects human and rat beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody
Detection of Human and Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 by Western Blot.
Western blot shows lysates of human brain tissue and rat testis tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for beta-1,3-N-acetylglucosaminyltransferase 2/B3GNT2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human brain tissue and rat testis tissue
Sample: Human brain tissue and rat testis tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2
B3-GNT (also B3GNT-2) is an 80 kDa (45 kDa without glycosylation) type II transmembrane Golgi enzyme that belongs to the glycosyltransferase 31 family of enzymes. It catalyzes the transfer of N-acetyl glucosamine from UDP-GlcNAc onto Gal beta 3(GlcNAc beta 6)GalNAc-mucin. It functions in the elongation and branching of O-linked oligosaccharide chains of mucin glycoproteins. Over aa 29-397, human B3GNT2 shares 88% aa sequence identity with rat B3GNT2.
Long Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyl-transferase 1
Alternate Names
B3Galt6, Beta3gnt1
Gene Symbol
B3GNT2
UniProt
Additional Beta-1,3-N-Acetylglucosaminyltransferase 2/B3GNT2 Products
Product Documents for Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody
Product Specific Notices for Human/Rat beta-1,3-N-acetylglucosaminyl-transferase 2/B3GNT2 Antibody
For research use only
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